WGS Upscaling - Pipeline Capacity

DRAEN pipeline capacity
Author
Affiliation

Xuyang Yuan

GDx OUSAMG

Published

February 12, 2025

1 Background

GDx at OUSAMG is planning to upscale the WGS production to:

  • 192 samples (4 proj x 48 or 2 proj x 48 + 1 proj x 96) per week
  • {r} 5.5*48 samples (2 fc x 48 + 3.5 fc x 48) per week
  • {r} 6.5*48 samples (2 fc x 48 + 4.5 fc x 48) per week

The capacity of IT & bioinformatics pipelines can be accessed in three key areas:

  1. Data transfer speed
  2. Data storage
  3. Pipeline capacity (this report)

2 Available hardware

Table 1: Available DRAGENs
Machine Units DRAGEN type DRAGENs per unit Total DRAGENs
NovaSeq X Plus 2a Onboard 4 8
NSC DRAGEN 2b External 1 2
TSD DRAGEN 1 External 1 1
  1. nox and nixie
  2. stormfly and toothless

3 Sequencing βž• Demultiplexing βž• NSC delivery data for bioinformatics pipeline

This section will evaluate the time needed for sequencing, demultiplexing and copying data from NovaSeq X to external storage (Boston).

3.1 Single 25B flowcell, 2nd Analysis 🟰 BCLConvert only

3.1.1 NovaSeq X reported time

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dateFormat YYYY-MM-DD HH:mm:ss
axisFormat %d/%b %H:%M
title NovaSeq X reported time
section single 25B flowcell<br/>(64 samples)
Sequencing completed 44h : 2024-11-18 13:02:04, 2024-11-20 06:51:37
Sequencing file transfer to external storage completed βž•2h: 2024-11-18 19:32:40, 2024-11-20 08:57:45
Analysis completed βž•5h: 2024-11-18 19:32:44, 2024-11-20 13:58:15
Analysis file transfer to external storage completed βž•0h: 2024-11-18 19:32:45, 2024-11-20 13:58:15
Figure 1: Single 25B flowcell 2nd Analysis BCL Convert only NovaSeq X reported time
Table 2: example run 20241118
Status Started Completed
Sequencing completed 2024-11-18 13:02:04 2024-11-20 06:51:37
Sequencing file transfer to external storage completed 2024-11-18 19:32:40 2024-11-20 08:57:45
Analysis completed 2024-11-18 19:32:44 2024-11-20 13:58:15
Analysis file transfer to external storage completed 2024-11-18 19:32:45 2024-11-20 13:58:15

3.1.2 Detailed steps time

The time taken by each step of each Novaseq X run differs. Numbers listed here is β€œclose to” the medians.

  • Sequencing of a single 25B flowcell takes 44h.
  • Sequencing file transfer to external storage starts 7h after sequencing start and finishes 2h after sequencing finishes.
    • Post-run wash time included which takes 1h48m
    • Some reports need to be copied to external storage. But they must wait for completion of post-run wash.
  • Secondary analysis (BCL Convert, Fastqc, ORA compression) takes extra 6h.
    • BCL Convert takes 2h after sequencing finish.
    • Fastqc and ORA compression take 4h after BCL Convert finish.
  • Coping secondary analysis data from sequencer to external storage (boston) takes extra 1h20m.
    • Copying fastq.ora files takes 1h
    • Copying other files, e.g., reports, logs, metadata files takes 20m
  • After copying data to boston complete, deliver sequencing data to the nscDeliver folder, calculating md5sum and push samples to lims-exporter queue takes extra 15m.

Sequencing starts to samples ready for running pipleline takes 52h.

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dateFormat YYYY-MM-DD HH:mm:ss
axisFormat %d/%b %H:%M
title NovaSeq X Sequenicing ➑  Ready for lims-exporter
section single 25B flowcell<br/>(64 samples)
Sequencing 44h: seq, 2024-11-18 13:02:04, 2024-11-20 06:51:37
bcl2fastq: 2024-11-19 21:12:28, 2024-11-19 23:35:36
32 sample ora compress: 2024-11-19 23:35:36, 2024-11-20 01:21:08
FastQC (map-align) msg_001: 2024-11-19 23:35:36, 2024-11-20 02:53:09
FastQC (map-align) msg_002: 2024-11-19 23:35:36, 2024-11-20 02:58:34
32 sample ora compress: 2024-11-20 01:21:08, 2024-11-20 03:08:53
FastQC report generation: 2024-11-20 10:54:00, 2024-11-20 11:11:00
Analysis file transfer to boston: 2024-11-20 13:40:00, 2024-11-20 14:00:00
NSC automation (md5sum+deliver): 2024-11-20 14:05:02, 2024-11-20 14:15:05

Table 3: Example run 20241118
Status Started Completed
Sequencing completed 2024-11-18 13:02:04 2024-11-20 06:51:37
bcl2fastq 2024-11-19 21:12:28 2024-11-19 23:35:36
32 sample ora compress 2024-11-19 23:35:36 2024-11-20 01:21:08
FastQC (map-align) msg_001 2024-11-19 23:35:36 2024-11-20 02:53:09
FastQC (map-align) msg_002 2024-11-19 23:35:36 2024-11-20 02:58:34
32 sample ora compress 2024-11-20 01:21:08 2024-11-20 03:08:53
FastQC report generation 2024-11-20 10:54:00 2024-11-20 11:11:00
Analysis file transfer to boston 2024-11-20 13:40:00 2024-11-20 14:00:00
NSC automation (md5sum+deliver) 2024-11-20 14:05:02 2024-11-20 14:15:05
Caution

This table shows the time for a single run on 2024-11-18.

3.2 Dual 25B flowcell; 2nd Analysis 🟰 BCL Convert only

The time taken by each step of each Novaseq X run differs. Numbers listed here is from a specific run 20241209.

3.2.1 NovaSeq X reported time

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dateFormat YYYY-MM-DD HH:mm:ss
axisFormat %d/%b %H:%M
title NovaSeq X reported time
section Flowcell B
Sequencing completed 44h :2024-12-09 11:30:41,2024-12-11 07:09:03
Sequencing file transfer to external storage completed βž•2h:2024-12-09 18:01:57,2024-12-11 09:17:40
Analysis completed βž•5h:2024-12-09 18:02:06,2024-12-11 14:03:25
Analysis file transfer to external storage completed βž•0h:2024-12-09 18:02:07,2024-12-11 14:03:25

section Flowcell A
Sequencing completed 44h :2024-12-09 11:30:37,2024-12-11 07:12:55
Sequencing file transfer to external storage completed βž•2h:2024-12-09 18:02:33,2024-12-11 09:22:38
Analysis completed βž•10h:2024-12-09 18:02:38,2024-12-11 19:28:08
Analysis file transfer to external storage completed βž•0h:2024-12-09 18:02:40,2024-12-11 19:28:08
Figure 2: NovaSeq X reported time, Dual 25B flowcell, 2nd Analysis BCL Convert only
Table 4: NovaSeq X reported time example run 20241209
(a) Flowcell B
Status Started Completed Duration
Sequencing completed 2024-12-09 11:30:41 2024-12-11 07:09:03 43h37m
Sequencing file transfer to external storage completed 2024-12-09 18:01:57 2024-12-11 09:17:40 39h15m
Analysis completed 2024-12-09 18:02:06 2024-12-11 14:03:25 44h01m
Analysis file transfer to external storage completed 2024-12-09 18:02:07 2024-12-11 14:03:25 44h01m
(b) Flowcell A
Status Started Completed Duration
Sequencing completed 2024-12-09 11:30:37 2024-12-11 07:12:55 43h42m
Sequencing file transfer to external storage completed 2024-12-09 18:02:33 2024-12-11 09:22:38 39h19m
Analysis completed 2024-12-09 18:02:38 2024-12-11 19:28:08 49h24m
Analysis file transfer to external storage completed 2024-12-09 18:02:40 2024-12-11 19:28:08 49h24m
Caution

This table shows the time for a single run on 20241209.

3.2.2 Detailed steps time

  • Sequencing of dual 25B flowcell takes 44h.
  • Sequencing file transfer to external storage starts 7h after sequencing start and finishes 2h after sequencing finishes.
    • Post-run wash time included which takes 1h48m
    • Some reports need to be copied to external storage. But they must wait for completion of post-run wash.
  • Secondary analysis (BCL Convert, Fastqc, ORA compression, FastQC reporting) takes extra 10h.
    • Flowcell B BCL Convert takes 2h for each flowcell separately.
    • Flowcell B Fastqc and ORA compression are done in parallel and finishes 3h after BCL Convert finish.
    • Flowcell A BCL Convert takes 2h for each flowcell separately.
    • Flowcell A BFastqc and ORA compression are done in parallel and finishes 3h after BCL Convert finish.
    • Flowcell B FastQC reporting for flowcell B takes 1h40m.
    • Flowcell A FastQC reporting for flowcell B takes 1h40m.
  • Coping secondary analysis data from sequencer to external storage (boston) takes extra 2h40m.
    • Copying fastq.ora files takes 1h
    • Copying other files, e.g., reports, logs, metadata files takes 20m
    • Sequencial for flowcell B, then A.
  • After copying data to boston complete, deliver sequencing data to the nscDeliver folder, calculating md5sum and push samples to lims-exporter queue takes extra 15m for each flowcell.

Sequencing starts to samples ready for running pipleline takes 57h.

3.2.2.1 Flowcell B Flowcell A 

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dateFormat YYYY-MM-DD HH:mm:ss
axisFormat %d/%b %H:%M
title NovaSeq X Sequenicing ➑  Ready for lims-exporter
section Flowcell B
RunStartTime (B): milestone, 2024-12-09 11:03:53, 0d
Sequencing 44h (B): seq, 2024-12-09 11:30:41, 2024-12-11 07:09:03
Instrument Analytics Cycle 1 - 320 (B): 2024-12-09 17:01:58, 2024-12-11 06:09:02
BCL CONVERSION B: convB, 2024-12-10 04:52:36, 2024-12-10 21:33:20
bcl2fastq (B): 2024-12-10 21:33:21, 2024-12-10 23:39:08
16 sample ora compress (B): 2024-12-10 23:39:09, 2024-12-11 00:40:27
16 sample ora compress (B): 2024-12-10 23:39:09, 2024-12-11 00:36:40
16 sample ora compress (B): 2024-12-10 23:39:09, 2024-12-11 00:47:16
FastQC (map-align) (B) msg_001: 2024-12-10 23:39:09, 2024-12-11 01:16:52
FastQC (map-align) (B) msg_002: 2024-12-10 23:39:09, 2024-12-11 02:49:01
FastQC (map-align) (B) msg_003: 2024-12-10 23:39:09, 2024-12-11 01:21:56
FastQC (map-align) (B) msg_004: 2024-12-10 23:39:09, 2024-12-11 01:21:20
16 sample ora compress (B): 2024-12-11 00:36:40, 2024-12-11 01:33:13
fastq generation complete (B): milestone, 2024-12-11 07:39:08, 0d
Post Run (B): 2024-12-11 07:47:48, 2024-12-11 09:00:31
RunEndTime (B): milestone, 2024-12-11 09:04:23, 0d
FastQC report generation (B): 2024-12-11 09:12:00, 2024-12-11 10:50:00
Analysis file transfer to boston (B): 2024-12-11 12:40:00, 2024-12-11 14:02:00
NSC automation (md5sum+deliver) (B):  2024-12-11 14:10:02, 2024-12-11 14:16:49
section Flowcell A
RunStartTime (A): milestone, 2024-12-09 11:03:52, 0d
Sequencing 44h (A): seq, 2024-12-09 11:30:37, 2024-12-11 07:12:55
Instrument Analytics Cycle 1 - 320 (A): 2024-12-09 17:02:34, 2024-12-11 06:12:54
BCL CONVERSION A: convA, 2024-12-10 04:49:52, 2024-12-10 21:29:01
bcl2fastq (A): 2024-12-11 02:51:04, 2024-12-11 04:54:52
16 sample ora compress (A): 2024-12-11 04:54:52, 2024-12-11 06:03:44
16 sample ora compress (A): 2024-12-11 04:54:52, 2024-12-11 06:00:23
16 sample ora compress (A): 2024-12-11 04:54:52, 2024-12-11 06:04:37
FastQC (map-align) (A) msg_001: 2024-12-11 04:54:52, 2024-12-11 08:11:00
FastQC (map-align) (A) msg_002: 2024-12-11 04:54:52, 2024-12-11 06:32:46
FastQC (map-align) (A) msg_003: 2024-12-11 04:54:52, 2024-12-11 06:34:32
FastQC (map-align) (A) msg_004: 2024-12-11 04:54:52, 2024-12-11 06:35:42
16 sample ora compress (A): 2024-12-11 06:00:23, 2024-12-11 06:57:40
Post Run (A): 2024-12-11 07:54:00, 2024-12-11 09:04:23
RunEndTime (A): milestone, 2024-12-11 09:04:23, 0d
fastq generation complete (A): milestone, 2024-12-11 12:54:52, 0d
FastQC report generation (A): 2024-12-11 14:33:00, 2024-12-11 16:12:00
Analysis file transfer to boston (A): 2024-12-11 18:00:00, 2024-12-11 19:27:00
NSC automation (md5sum+deliver) (A):  2024-12-11 19:35:01,  2024-12-11 19:42:11
Figure 3: Dual 25B flowcell 2nd Analysis BCL Convert only NovaSeq X reported time
Table 5: Dual 25B flowcell, 2nd Analysis BCL Convert only, detailed steps time, run 20241209
(a) Flowcell B
Job Started Completed
RunStartTime (B) 2024-12-09 11:03:53
Sequencing completed (B) 2024-12-09 11:30:41 2024-12-11 07:09:03
Instrument Analytics Cycle 1 - 320 (B) 2024-12-09 17:01:58 2024-12-11 06:09:02
BCL CONVERSION B 2024-12-10 04:52:36 2024-12-10 21:33:20
bcl2fastq (B) 2024-12-10 21:33:21 2024-12-10 23:39:08
16 sample ora compress (B) 2024-12-10 23:39:09 2024-12-11 00:40:27
16 sample ora compress (B) 2024-12-10 23:39:09 2024-12-11 00:36:40
16 sample ora compress (B) 2024-12-10 23:39:09 2024-12-11 00:47:16
FastQC (map-align) (B) msg_001 2024-12-10 23:39:09 2024-12-11 01:16:52
FastQC (map-align) (B) msg_002 2024-12-10 23:39:09 2024-12-11 02:49:01
FastQC (map-align) (B) msg_003 2024-12-10 23:39:09 2024-12-11 01:21:56
FastQC (map-align) (B) msg_004 2024-12-10 23:39:09 2024-12-11 01:21:20
16 sample ora compress (B) 2024-12-11 00:36:40 2024-12-11 01:33:13
fastq generation complete (B) 2024-12-11 07:39:08
Post Run (B) 2024-12-11 07:47:48 2024-12-11 09:00:31
RunEndTime (B) 2024-12-11 09:04:23
FastQC report generation (B) 2024-12-11 09:12:00 2024-12-11 10:50:00
Analysis file transfer to boston (B) 2024-12-11 12:40:00 2024-12-11 14:02:00
NSC automation (md5sum+deliver) (B) 2024-12-11 14:10:02 2024-12-11 14:16:49
(b) Flowcell A
Job Started Completed
RunStartTime (A) 2024-12-09 11:03:52
Sequencing completed (A) 2024-12-09 11:30:37 2024-12-11 07:12:55
Instrument Analytics Cycle 1 - 320 (A) 2024-12-09 17:02:34 2024-12-11 06:12:54
BCL CONVERSION A 2024-12-10 04:49:52 2024-12-10 21:29:01
bcl2fastq (A) 2024-12-11 02:51:04 2024-12-11 04:54:52
16 sample ora compress (A) 2024-12-11 04:54:52 2024-12-11 06:03:44
16 sample ora compress (A) 2024-12-11 04:54:52 2024-12-11 06:00:23
16 sample ora compress (A) 2024-12-11 04:54:52 2024-12-11 06:04:37
FastQC (map-align) (A) msg_001 2024-12-11 04:54:52 2024-12-11 08:11:00
FastQC (map-align) (A) msg_002 2024-12-11 04:54:52 2024-12-11 06:32:46
FastQC (map-align) (A) msg_003 2024-12-11 04:54:52 2024-12-11 06:34:32
FastQC (map-align) (A) msg_004 2024-12-11 04:54:52 2024-12-11 06:35:42
16 sample ora compress (A) 2024-12-11 06:00:23 2024-12-11 06:57:40
Post Run (A) 2024-12-11 07:54:00 2024-12-11 09:04:23
RunEndTime (A) 2024-12-11 09:04:23
fastq generation complete (A) 2024-12-11 12:54:52
FastQC report generation (A) 2024-12-11 14:33:00 2024-12-11 16:12:00
Analysis file transfer to boston (A) 2024-12-11 18:00:00 2024-12-11 19:27:00
NSC automation (md5sum+deliver) (A) 2024-12-11 19:35:01 2024-12-11 19:42:11
Caution

This table shows the time for a single run on 2024-12-09.

3.2.2.2 Time sorted 

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dateFormat YYYY-MM-DD HH:mm:ss
axisFormat %d/%b %H:%M
title NovaSeq X Sequenicing ➑  Ready for lims-exporter
RunStartTime (A): milestone, 2024-12-09 11:03:52, 0d
RunStartTime (B): milestone, 2024-12-09 11:03:53, 0d
Sequencing 44h (A): seq, 2024-12-09 11:30:37, 2024-12-11 07:12:55
Sequencing 44h (B): seq, 2024-12-09 11:30:41, 2024-12-11 07:09:03
Instrument Analytics Cycle 1 - 320 (B): 2024-12-09 17:01:58, 2024-12-11 06:09:02
Instrument Analytics Cycle 1 - 320 (A): 2024-12-09 17:02:34, 2024-12-11 06:12:54
BCL CONVERSION A: convA, 2024-12-10 04:49:52, 2024-12-10 21:29:01
BCL CONVERSION B: convB, 2024-12-10 04:52:36, 2024-12-10 21:33:20
bcl2fastq (B): 2024-12-10 21:33:21, 2024-12-10 23:39:08
16 sample ora compress (B): 2024-12-10 23:39:09, 2024-12-11 00:36:40
16 sample ora compress (B): 2024-12-10 23:39:09, 2024-12-11 00:40:27
16 sample ora compress (B): 2024-12-10 23:39:09, 2024-12-11 00:47:16
FastQC (map-align) (B) msg_001: 2024-12-10 23:39:09, 2024-12-11 01:16:52
FastQC (map-align) (B) msg_004: 2024-12-10 23:39:09, 2024-12-11 01:21:20
FastQC (map-align) (B) msg_003: 2024-12-10 23:39:09, 2024-12-11 01:21:56
FastQC (map-align) (B) msg_002: 2024-12-10 23:39:09, 2024-12-11 02:49:01
16 sample ora compress (B): 2024-12-11 00:36:40, 2024-12-11 01:33:13
bcl2fastq (A): 2024-12-11 02:51:04, 2024-12-11 04:54:52
16 sample ora compress (A): 2024-12-11 04:54:52, 2024-12-11 06:00:23
16 sample ora compress (A): 2024-12-11 04:54:52, 2024-12-11 06:03:44
16 sample ora compress (A): 2024-12-11 04:54:52, 2024-12-11 06:04:37
FastQC (map-align) (A) msg_002: 2024-12-11 04:54:52, 2024-12-11 06:32:46
FastQC (map-align) (A) msg_003: 2024-12-11 04:54:52, 2024-12-11 06:34:32
FastQC (map-align) (A) msg_004: 2024-12-11 04:54:52, 2024-12-11 06:35:42
FastQC (map-align) (A) msg_001: 2024-12-11 04:54:52, 2024-12-11 08:11:00
16 sample ora compress (A): 2024-12-11 06:00:23, 2024-12-11 06:57:40
fastq generation complete (B): milestone, 2024-12-11 07:39:08, 0d
Post Run (B): 2024-12-11 07:47:48, 2024-12-11 09:00:31
Post Run (A): 2024-12-11 07:54:00, 2024-12-11 09:04:23
RunEndTime (B): milestone, 2024-12-11 09:04:23, 0d
RunEndTime (A): milestone, 2024-12-11 09:04:23, 0d
FastQC report generation (B): 2024-12-11 09:12:00, 2024-12-11 10:50:00
Analysis file transfer to boston (B): 2024-12-11 12:40:00, 2024-12-11 14:02:00
fastq generation complete (A): milestone, 2024-12-11 12:54:52, 0d
NSC automation (md5sum+deliver) (B):  2024-12-11 14:10:02, 2024-12-11 14:16:49
FastQC report generation (A): 2024-12-11 14:33:00, 2024-12-11 16:12:00
Analysis file transfer to boston (A): 2024-12-11 18:00:00, 2024-12-11 19:27:00
NSC automation (md5sum+deliver) (A):  2024-12-11 19:35:01,  2024-12-11 19:42:11
Figure 4: Dual 25B flowcell 2nd Analysis BCL Convert only NovaSeq X reported time
Table 6: Dual 25B flowcell, 2nd Analysis BCL Convert only, detailed steps time, time sorted, run 20241209 {style='font-size: 0.7em;'}
Job Started Completed
RunStartTime (A) 2024-12-09 11:03:52
RunStartTime (B) 2024-12-09 11:03:53
Sequencing completed (A) 2024-12-09 11:30:37 2024-12-11 07:12:55
Sequencing completed (B) 2024-12-09 11:30:41 2024-12-11 07:09:03
Instrument Analytics Cycle 1 - 320 (B) 2024-12-09 17:01:58 2024-12-11 06:09:02
Instrument Analytics Cycle 1 - 320 (A) 2024-12-09 17:02:34 2024-12-11 06:12:54
BCL CONVERSION A 2024-12-10 04:49:52 2024-12-10 21:29:01
BCL CONVERSION B 2024-12-10 04:52:36 2024-12-10 21:33:20
bcl2fastq (B) 2024-12-10 21:33:21 2024-12-10 23:39:08
16 sample ora compress (B) 2024-12-10 23:39:09 2024-12-11 00:36:40
16 sample ora compress (B) 2024-12-10 23:39:09 2024-12-11 00:40:27
16 sample ora compress (B) 2024-12-10 23:39:09 2024-12-11 00:47:16
FastQC (map-align) (B) msg_001 2024-12-10 23:39:09 2024-12-11 01:16:52
FastQC (map-align) (B) msg_004 2024-12-10 23:39:09 2024-12-11 01:21:20
FastQC (map-align) (B) msg_003 2024-12-10 23:39:09 2024-12-11 01:21:56
FastQC (map-align) (B) msg_002 2024-12-10 23:39:09 2024-12-11 02:49:01
16 sample ora compress (B) 2024-12-11 00:36:40 2024-12-11 01:33:13
bcl2fastq (A) 2024-12-11 02:51:04 2024-12-11 04:54:52
16 sample ora compress (A) 2024-12-11 04:54:52 2024-12-11 06:00:23
16 sample ora compress (A) 2024-12-11 04:54:52 2024-12-11 06:03:44
16 sample ora compress (A) 2024-12-11 04:54:52 2024-12-11 06:04:37
FastQC (map-align) (A) msg_002 2024-12-11 04:54:52 2024-12-11 06:32:46
FastQC (map-align) (A) msg_003 2024-12-11 04:54:52 2024-12-11 06:34:32
FastQC (map-align) (A) msg_004 2024-12-11 04:54:52 2024-12-11 06:35:42
FastQC (map-align) (A) msg_001 2024-12-11 04:54:52 2024-12-11 08:11:00
16 sample ora compress (A) 2024-12-11 06:00:23 2024-12-11 06:57:40
fastq generation complete (B) 2024-12-11 07:39:08
Post Run (B) 2024-12-11 07:47:48 2024-12-11 09:00:31
Post Run (A) 2024-12-11 07:54:00 2024-12-11 09:04:23
RunEndTime (B) 2024-12-11 09:04:23
RunEndTime (A) 2024-12-11 09:04:23
FastQC report generation (B) 2024-12-11 09:12:00 2024-12-11 10:50:00
Analysis file transfer to boston (B) 2024-12-11 12:40:00 2024-12-11 14:02:00
fastq generation complete (A) 2024-12-11 12:54:52
NSC automation (md5sum+deliver) (B) 2024-12-11 14:10:02 2024-12-11 14:16:49
FastQC report generation (A) 2024-12-11 14:33:00 2024-12-11 16:12:00
Analysis file transfer to boston (A) 2024-12-11 18:00:00 2024-12-11 19:27:00
NSC automation (md5sum+deliver) (A) 2024-12-11 19:35:01 2024-12-11 19:42:11
Caution

This table shows the time for a single run on 2024-12-09.

3.3 Dual 25B flowcell; 2nd Analysis 🟰 WGS Germline

Dual 25B flowcell (128 samples). 2nd Analysis is WGS Germline (mapping and variant calling). The time taken by each step of each Novaseq X run differs. Numbers listed here is from a specific run 20241213.

3.3.1 NovaSeq X reported time

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dateFormat YYYY-MM-DD HH:mm:ss
axisFormat %d/%b %H:%M
title NovaSeq X reported time
section Flowcell B
Sequencing completed 44h :2024-12-13 15:49:42, 2024-12-15 11:49:42
Sequencing file transfer to external storage completed βž•2h:2024-12-13 22:49:42, 2024-12-15 13:48:00
Analysis completed βž•12h:2024-12-13 22:49:42, 2024-12-16 01:47:00
Analysis file transfer to external storage completed βž•0h:2024-12-13 22:49:42,  2024-12-16 01:47:00

section Flowcell A
Sequencing completed 44h :2024-12-13 15:49:42, 2024-12-15 11:49:42
Sequencing file transfer to external storage completed βž•2h:2024-12-13 22:49:42, 2024-12-15 13:48:00
Analysis completed βž•22h:2024-12-13 22:49:42, 2024-12-16 11:42:00
Analysis file transfer to external storage completed βž•0h:2024-12-13 22:49:42,  2024-12-16 11:42:00
Figure 5

3.3.2 Detailed steps time

  • Sequencing of dual 25B flowcell takes 44h.
  • Sequencing file transfer to external storage starts 7h after sequencing start and finishes 2h after sequencing finishes.
    • Post-run wash time included which takes 1h48m
    • Some reports need to be copied to external storage. But they must wait for completion of post-run wash.
  • Secondary analysis (BCL Convert, Fastqc, ORA compression, Mapping and all variant calling) takes extra 16h.
  • Coping secondary analysis data from sequencer to external storage (boston) takes extra 6h
    • 3h for flowcell B
    • 3h for flowcell A
  • After copying data to boston complete, deliver sequencing data to the nscDeliver folder, calculating md5sum and push samples to lims-exporter queue takes extra 15m for each flowcell.

Sequencing starts to samples ready for running pipleline takes 68h.

3.3.2.1 Flowcell B Flowcell A 

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dateFormat YYYY-MM-DD HH:mm:ss
axisFormat %d/%b %H:%M
title NovaSeq X Sequenicing ➑  Ready for lims-exporter
section Flowcell B
RunStartTime (B): milestone, 2024-12-13 15:22:38, 0d
Instrument Analytics Cycle 1 - 320 (B): 2024-12-13 21:32:07, 2024-12-15 10:39:45 
BCL CONVERSION (B): 2024-12-14 09:20:26, 2024-12-15 01:58:22
bcl2fastq (B): 2024-12-15 04:41:06, 2024-12-15 04:55:58
Post Run (B): 2024-12-15 11:39:46, 2024-12-15 13:31:14
RunEndTime (B): milestone, 2024-12-15 13:35:06, 0d
8 sample pipeline (B)(msg_001): 2024-12-15 02:14:40,  2024-12-15 06:12:10
8 sample pipeline (B)(msg_002): 2024-12-15 02:16:03,  2024-12-15 06:08:38
8 sample pipeline (B)(msg_003): 2024-12-15 02:21:34,  2024-12-15 06:02:43
(waiting for board access 1.7h) 8 sample pipeline (B)(msg_004): 2024-12-15 02:30:31,  2024-12-15 07:21:59
8 sample pipeline (B)(msg_005): 2024-12-15 06:02:54,  2024-12-15 09:27:04
8 sample pipeline (B)(msg_006): 2024-12-15 06:09:01,  2024-12-15 10:15:17
(waiting for board access 1.2h) 8 sample pipeline (B)(msg_007): 2024-12-15 06:12:43,  2024-12-15 11:00:17
(waiting for board access 2h) 8 sample pipeline (B)(msg_008): 2024-12-15 07:22:41,  2024-12-15 12:21:11
FastQC etc. report generation (B): 2024-12-15 17:33:00, 2024-12-15 20:27:00
Analysis file transfer to boston (B): 2024-12-15 22:10:00, 2024-12-16 01:10:00
NSC automation (md5sum+deliver) (B):  2024-12-16 01:15:01, 2024-12-16 01:22:02
section Flowcell A
RunStartTime (A): milestone, 2024-12-13 15:22:39, 0d
Instrument Analytics Cycle 1 - 320 (A): 2024-12-13 21:32:38, 2024-12-15 10:43:37
BCL CONVERSION (A): 2024-12-14 09:23:44, 2024-12-15 02:03:05
Post Run (A): 2024-12-15 11:43:38, 2024-12-15 13:35:06
RunEndTime (A): milestone, 2024-12-15 13:35:06, 0d
bcl2fastq (A): 2024-12-15 15:36:32, 2024-12-15 15:55:22
8 sample pipeline (A) (msg_001): 2024-12-15 12:35:29,  2024-12-15 16:20:28
8 sample pipeline (A) (msg_002): 2024-12-15 12:41:54,  2024-12-15 15:59:51
8 sample pipeline (A) (msg_003): 2024-12-15 12:46:44,  2024-12-15 16:29:28
(waiting for board access 3h) 8 sample pipeline (A) (msg_004): 2024-12-15 12:57:58,  2024-12-15 19:40:46
8 sample pipeline (A) (msg_005): 2024-12-15 15:59:59,  2024-12-15 20:09:04
8 sample pipeline (A) (msg_006): 2024-12-15 16:21:01,  2024-12-15 20:12:05
8 sample pipeline (A) (msg_007): 2024-12-15 16:29:32,  2024-12-15 20:44:58
8 sample pipeline (A) (msg_008): 2024-12-15 19:40:54,  2024-12-15 22:53:59
FastQC etc. report generation (A): 2024-12-16 04:20:00, 2024-12-16 07:00:00
Analysis file transfer to boston (A): 2024-12-16 08:40:00, 2024-12-16 11:40:00
NSC automation (md5sum+deliver) (A):  2024-12-16 11:50:01, 2024-12-16 11:56:58
Figure 6: Dual 25B flowcell 2nd Analysis BCL Convert only NovaSeq X reported time
Table 7: Dual 25B flowcell, 2nd Analysis BCL Convert only, detailed steps time, run 20241209
(a) Flowcell B
Job Started Completed
RunStartTime (B) 2024-12-13 15:22:38
Instrument Analytics Cycle 1 - 320 (B) 2024-12-13 21:32:07 2024-12-15 10:39:45
BCL CONVERSION (B) 2024-12-14 09:20:26 2024-12-15 01:58:22
Post Run (B) 2024-12-15 11:39:46 2024-12-15 13:31:14
RunEndTime (B) 2024-12-15 13:35:06
8 sample pipeline (msg_001) 2024-12-15 02:14:40 2024-12-15 06:12:10
8 sample pipeline (msg_002) 2024-12-15 02:16:03 2024-12-15 06:08:38
8 sample pipeline (msg_003) 2024-12-15 02:21:34 2024-12-15 06:02:43
8 sample pipeline (msg_004) 2024-12-15 02:30:31 2024-12-15 07:21:59
8 sample pipeline (msg_005) 2024-12-15 06:02:54 2024-12-15 09:27:04
8 sample pipeline (msg_006) 2024-12-15 06:09:01 2024-12-15 10:15:17
8 sample pipeline (msg_007) 2024-12-15 06:12:43 2024-12-15 11:00:17
8 sample pipeline (msg_008) 2024-12-15 07:22:41 2024-12-15 12:21:11
FastQC etc. report generation (B) 2024-12-15 17:33:00 2024-12-15 20:27:00
Analysis file transfer to boston (B) 2024-12-15 22:10:00 2024-12-16 01:10:00
NSC automation (md5sum+deliver) (B) 2024-12-16 01:15:01 2024-12-16 01:22:02
(b) Flowcell A
Job Started Completed
RunStartTime (A) 2024-12-13 15:22:39
Instrument Analytics Cycle 1 - 320 (A) 2024-12-13 21:32:38 2024-12-15 10:43:37
Post Run (A) 2024-12-15 11:43:38 2024-12-15 13:35:06
RunEndTime (A) 2024-12-15 13:35:06
BCL CONVERSION (A) 2024-12-14 09:23:44 2024-12-15 02:03:05
8 sample pipeline(msg_001) 2024-12-15 12:35:29 2024-12-15 16:20:28
8 sample pipeline(msg_002) 2024-12-15 12:41:54 2024-12-15 15:59:51
8 sample pipeline(msg_003) 2024-12-15 12:46:44 2024-12-15 16:29:28
(waiting for board access 3h) 8 sample pipeline(msg_004) 2024-12-15 12:57:58 2024-12-15 19:40:46
8 sample pipeline(msg_005) 2024-12-15 15:59:59 2024-12-15 20:09:04
8 sample pipeline(msg_006) 2024-12-15 16:21:01 2024-12-15 20:12:05
8 sample pipeline(msg_007) 2024-12-15 16:29:32 2024-12-15 20:44:58
8 sample pipeline(msg_008) 2024-12-15 19:40:54 2024-12-15 22:53:59
FastQC etc. report generation (A) 2024-12-16 04:20:00 2024-12-16 07:00:00
Analysis file transfer to boston (A) 2024-12-16 08:40:00 2024-12-16 11:40:00
NSC automation (md5sum+deliver) (A) 2024-12-16 11:50:01 2024-12-16 11:56:58
Caution

This table shows the time for a single run on 2024-12-09.

3.3.2.2 Time sorted 

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dateFormat YYYY-MM-DD HH:mm:ss
axisFormat %d/%b %H:%M
title NovaSeq X Sequenicing ➑  Ready for lims-exporter
RunStartTime (B): milestone, 2024-12-13 15:22:38, 0d
RunStartTime (A): milestone, 2024-12-13 15:22:39, 0d
Instrument Analytics Cycle 1 - 320 (B): 2024-12-13 21:32:07, 2024-12-15 10:39:45 
Instrument Analytics Cycle 1 - 320 (A): 2024-12-13 21:32:38, 2024-12-15 10:43:37
BCL CONVERSION (B): 2024-12-14 09:20:26, 2024-12-15 01:58:22
BCL CONVERSION (A): 2024-12-14 09:23:44, 2024-12-15 02:03:05
8 sample pipeline (B)(msg_001): 2024-12-15 02:14:40,  2024-12-15 06:12:10
8 sample pipeline (B)(msg_002): 2024-12-15 02:16:03,  2024-12-15 06:08:38
8 sample pipeline (B)(msg_003): 2024-12-15 02:21:34,  2024-12-15 06:02:43
(waiting for board access 1.7h) 8 sample pipeline (B)(msg_004): 2024-12-15 02:30:31,  2024-12-15 07:21:59
bcl2fastq (B): 2024-12-15 04:41:06, 2024-12-15 04:55:58
8 sample pipeline (B)(msg_005): 2024-12-15 06:02:54,  2024-12-15 09:27:04
8 sample pipeline (B)(msg_006): 2024-12-15 06:09:01,  2024-12-15 10:15:17
(waiting for board access 1.2h) 8 sample pipeline (B)(msg_007): 2024-12-15 06:12:43,  2024-12-15 11:00:17
(waiting for board access 2h) 8 sample pipeline (B)(msg_008): 2024-12-15 07:22:41,  2024-12-15 12:21:11
Post Run (B): 2024-12-15 11:39:46, 2024-12-15 13:31:14
Post Run (A): 2024-12-15 11:43:38, 2024-12-15 13:35:06
8 sample pipeline (A) (msg_001): 2024-12-15 12:35:29,  2024-12-15 16:20:28
8 sample pipeline (A) (msg_002): 2024-12-15 12:41:54,  2024-12-15 15:59:51
8 sample pipeline (A) (msg_003): 2024-12-15 12:46:44,  2024-12-15 16:29:28
(waiting for board access 3h) 8 sample pipeline (A) (msg_004): 2024-12-15 12:57:58,  2024-12-15 19:40:46
RunEndTime (B): milestone, 2024-12-15 13:35:06, 0d
RunEndTime (A): milestone, 2024-12-15 13:35:06, 0d
bcl2fastq (A): 2024-12-15 15:36:32, 2024-12-15 15:55:22
8 sample pipeline (A) (msg_005): 2024-12-15 15:59:59,  2024-12-15 20:09:04
8 sample pipeline (A) (msg_006): 2024-12-15 16:21:01,  2024-12-15 20:12:05
8 sample pipeline (A) (msg_007): 2024-12-15 16:29:32,  2024-12-15 20:44:58
FastQC etc. report generation (B): 2024-12-15 17:33:00, 2024-12-15 20:27:00
8 sample pipeline (A) (msg_008): 2024-12-15 19:40:54,  2024-12-15 22:53:59
Analysis file transfer to boston (B): 2024-12-15 22:10:00, 2024-12-16 01:10:00
NSC automation (md5sum+deliver) (B):  2024-12-16 01:15:01, 2024-12-16 01:22:02
FastQC etc. report generation (A): 2024-12-16 04:20:00, 2024-12-16 07:00:00
Analysis file transfer to boston (A): 2024-12-16 08:40:00, 2024-12-16 11:40:00
NSC automation (md5sum+deliver) (A):  2024-12-16 11:50:01, 2024-12-16 11:56:58
Figure 7: Dual 25B flowcell 2nd Analysis BCL Convert only NovaSeq X reported time
Table 8: Dual 25B flowcell, 2nd Analysis BCL Convert only, detailed steps time, run 20241209
Job Started Completed
RunStartTime (B) 2024-12-13 15:22:38
RunStartTime (A) 2024-12-13 15:22:39
Instrument Analytics Cycle 1 - 320 (B) 2024-12-13 21:32:07 2024-12-15 10:39:45
Instrument Analytics Cycle 1 - 320 (A) 2024-12-13 21:32:38 2024-12-15 10:43:37
BCL CONVERSION (B) 2024-12-14 09:20:26 2024-12-15 01:58:22
BCL CONVERSION (A) 2024-12-14 09:23:44 2024-12-15 02:03:05
8 sample pipeline (B)(msg_001) 2024-12-15 02:14:40 2024-12-15 06:12:10
8 sample pipeline (B)(msg_002) 2024-12-15 02:16:03 2024-12-15 06:08:38
8 sample pipeline (B)(msg_003) 2024-12-15 02:21:34 2024-12-15 06:02:43
8 sample pipeline (B)(msg_004) 2024-12-15 02:30:31 2024-12-15 07:21:59
8 sample pipeline (B)(msg_005) 2024-12-15 06:02:54 2024-12-15 09:27:04
8 sample pipeline (B)(msg_006) 2024-12-15 06:09:01 2024-12-15 10:15:17
8 sample pipeline (B)(msg_007) 2024-12-15 06:12:43 2024-12-15 11:00:17
8 sample pipeline (B)(msg_008) 2024-12-15 07:22:41 2024-12-15 12:21:11
Post Run (B) 2024-12-15 11:39:46 2024-12-15 13:31:14
Post Run (A) 2024-12-15 11:43:38 2024-12-15 13:35:06
8 sample pipeline (A)(msg_001) 2024-12-15 12:35:29 2024-12-15 16:20:28
8 sample pipeline (A)(msg_002) 2024-12-15 12:41:54 2024-12-15 15:59:51
8 sample pipeline (A)(msg_003) 2024-12-15 12:46:44 2024-12-15 16:29:28
(waiting for board access 3h) 8 sample pipeline (A)(msg_004) 2024-12-15 12:57:58 2024-12-15 19:40:46
RunEndTime (B) 2024-12-15 13:35:06
RunEndTime (A) 2024-12-15 13:35:06
8 sample pipeline (A)(msg_005) 2024-12-15 15:59:59 2024-12-15 20:09:04
8 sample pipeline (A)(msg_006) 2024-12-15 16:21:01 2024-12-15 20:12:05
8 sample pipeline (A)(msg_007) 2024-12-15 16:29:32 2024-12-15 20:44:58
FastQC etc. report generation (B) 2024-12-15 17:33:00 2024-12-15 20:27:00
8 sample pipeline (A)(msg_008) 2024-12-15 19:40:54 2024-12-15 22:53:59
Analysis file transfer to boston (B) 2024-12-15 22:10:00 2024-12-16 01:10:00
NSC automation (md5sum+deliver) (B) 2024-12-16 01:15:01 2024-12-16 01:22:02
FastQC etc. report generation (A) 2024-12-16 04:20:00 2024-12-16 07:00:00
Analysis file transfer to boston (A) 2024-12-16 08:40:00 2024-12-16 11:40:00
NSC automation (md5sum+deliver) (A) 2024-12-16 11:50:01 2024-12-16 11:56:58
Caution

This table shows the time for a single run on 2024-12-09.

4 Pipeline capacity evaluation

DRAGEN is β€œsingle-threaded”. The pipeline capacity is determined by the number of DRAGENs available. And the time needed to process a sample.

4.1 Pipeline real-time monitoring (1 or 2 NSC DRAGEN in use)

With 1 (or 2) NSC DRAGENs in use, the pipeline can process up to 36 (or 72) samples in 24 hours.

4.1.1 2024-11-10 (Sun)

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β˜€: milestone, start, 2024-10-10 00:00:00.000, 0d
wgs418_61 (35m 2s): 2024-10-10 09:01:56.207,2024-10-10 09:37:16.310
wgs418_22 (30m 48s): 2024-10-10 09:41:32.212,2024-10-10 10:12:42.295
wgs418_29 (36m 51s): 2024-10-10 10:12:47.722,2024-10-10 10:49:47.793
wgs418_64 (29m 8s): 2024-10-10 11:31:49.234,2024-10-10 12:01:14.283
wgs418_55 (32m 36s): 2024-10-10 12:00:58.289,2024-10-10 12:33:58.354
wgs418_26 (36m 48s): 2024-10-10 12:33:54.663,2024-10-10 13:10:44.726
wgs418_47 (40m 5s): 2024-10-10 13:16:54.469,2024-10-10 13:56:34.552
wgs418_20 (33m 28s): 2024-10-10 13:56:24.638,2024-10-10 14:30:04.762
wgs418_31 (43m 44s): 2024-10-10 14:30:05.113,2024-10-10 15:13:50.243
wgs418_53 (32m 59s): 2024-10-10 15:13:50.820,2024-10-10 15:47:05.876
wgs418_42 (33m 49s): 2024-10-10 15:47:16.028,2024-10-10 16:20:51.089
wgs418_5 (32m 56s): 2024-10-10 16:20:28.782,2024-10-10 16:53:38.833
wgs418_52 (33m 17s): 2024-10-10 16:53:25.901,2024-10-10 17:26:40.954
wgs418_9 (33m 2s): 2024-10-10 17:58:03.609,2024-10-10 18:31:23.666
wgs418_4 (37m 14s): 2024-10-10 18:31:18.664,2024-10-10 19:08:38.789
wgs418_11 (36m 52s): 2024-10-10 19:08:20.053,2024-10-10 19:45:40.107
wgs418_18 (36m 29s): 2024-10-10 19:45:21.272,2024-10-10 20:22:11.325
wgs418_49 (31m 33s): 2024-10-10 20:22:30.249,2024-10-10 20:53:20.302
wgs418_7 (35m 29s): 2024-10-10 20:53:20.880,2024-10-10 21:29:00.945
wgs418_39 (32m 25s): 2024-10-10 21:28:50.833,2024-10-10 22:01:25.893
wgs418_1 (36m 55s): 2024-10-10 22:01:17.617,2024-10-10 22:38:27.682
wgs418_15 (34m 55s): 2024-10-10 22:38:37.089,2024-10-10 23:13:22.178
wgs418_8 (32m 44s): 2024-10-10 23:13:12.620,2024-10-10 23:46:02.741
wgs418_48 (38m 46s): 2024-10-10 23:45:52.665,2024-10-11 00:25:02.730
β˜ͺ: milestone, end, 2024-10-10 23:59:59.999, 0d

4.1.2 2024-11-11 (Mon)

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wgs418_12 (31m 1s): 2024-10-11 00:24:57.798,2024-10-11 00:55:47.861
wgs418_17 (37m 13s): 2024-10-11 00:55:53.641,2024-10-11 01:33:03.707
wgs418_58 (34m 35s): 2024-10-11 01:33:33.508,2024-10-11 02:07:28.627
wgs418_10 (31m 16s): 2024-10-11 02:07:48.762,2024-10-11 02:39:03.864
wgs418_2 (39m 54s): 2024-10-11 02:39:03.781,2024-10-11 03:18:38.846
wgs418_28 (31m 1s): 2024-10-11 03:19:10.487,2024-10-11 03:49:45.637
wgs418_14 (31m 13s): 2024-10-11 03:49:40.512,2024-10-11 04:20:55.566
wgs418_41 (31m 43s): 2024-10-11 04:21:05.627,2024-10-11 04:52:40.669
wgs418_59 (40m 24s): 2024-10-11 04:52:42.723,2024-10-11 05:33:22.805
wgs418_19 (32m 8s): 2024-10-11 05:33:08.295,2024-10-11 06:05:43.342
wgs418_35 (32m 42s): 2024-10-11 06:05:54.960,2024-10-11 06:37:55.015
wgs418_54 (33m 42s): 2024-10-11 06:37:53.247,2024-10-11 07:11:48.373
wgs418_13 (39m 31s): 2024-10-11 07:12:08.798,2024-10-11 07:51:08.854
wgs418_34 (30m 43s): 2024-10-11 07:51:14.392,2024-10-11 08:22:09.435
wgs418_36 (31m 46s): 2024-10-11 08:22:19.203,2024-10-11 08:53:39.251
wgs418_50 (37m 6s): 2024-10-11 08:54:24.281,2024-10-11 09:30:59.417
wgs418_33 (36m 58s): 2024-10-11 10:03:37.020,2024-10-11 10:39:52.084
wgs418_60 (44m 59s): 2024-10-11 10:39:51.746,2024-10-11 11:25:16.821
wgs418_16 (41m 41s): 2024-10-11 12:02:17.011,2024-10-11 12:44:02.077
wgs418_44 (44m 52s): 2024-10-11 12:44:22.181,2024-10-11 13:29:02.340
wgs418_51 (45m 28s): 2024-10-11 13:29:06.517,2024-10-11 14:14:41.592
wgs418_32 (41m 33s): 2024-10-11 14:14:27.425,2024-10-11 14:55:57.494
wgs418_38 (7m 5s, failed): crit, 2024-10-11 15:26:47.890,2024-10-11 15:33:52.898
wgs418_23 (7m 5s, failed): crit, 2024-10-11 15:26:49.670,2024-10-11 15:33:54.691
wgs418_57 (7m 5s, failed): crit, 2024-10-11 15:26:49.910,2024-10-11 15:33:54.942
wgs418_56 (7m 5s, failed): crit, 2024-10-11 15:26:56.506,2024-10-11 15:34:01.531
wgs418_24 (7m 5s, failed): crit, 2024-10-11 15:26:58.376,2024-10-11 15:34:03.388
wgs418_40 (7m 5s, failed): crit, 2024-10-11 15:26:59.629,2024-10-11 15:34:04.636
wgs418_37 (7m 5s, failed): crit, 2024-10-11 15:27:01.986,2024-10-11 15:34:06.997
wgs418_43 (7m 5s, failed): crit, 2024-10-11 15:27:13.990,2024-10-11 15:34:19.005
wgs418_21 (7m 5s, failed): crit, 2024-10-11 15:27:14.794,2024-10-11 15:34:19.798
wgs418_38 (38m 3s): 2024-10-11 15:33:57.887,2024-10-11 16:12:22.960
wgs418_23 (38m 59s): 2024-10-11 15:34:29.669,2024-10-11 16:13:44.855
wgs418_57 (30m 39s): 2024-10-11 16:12:34.992,2024-10-11 16:42:55.043
wgs418_56 (35m 59s): 2024-10-11 16:13:26.587,2024-10-11 16:49:31.658
wgs418_24 (37m 32s): 2024-10-11 16:42:48.506,2024-10-11 17:20:23.643
wgs418_40 (38m): 2024-10-11 16:49:29.757,2024-10-11 17:27:34.825
wgs418_37 (40m 2s): 2024-10-11 17:20:47.213,2024-10-11 18:00:22.364
wgs418_43 (29m 21s): 2024-10-11 17:28:09.193,2024-10-11 17:56:59.424
wgs418_21 (34m 39s): 2024-10-11 17:57:20.055,2024-10-11 18:31:35.121
wgs418_25 (31m 57s): 2024-10-11 18:00:16.227,2024-10-11 18:32:41.280
β˜ͺ: milestone, end, 2024-10-11 23:59:59.999, 0d

4.1.3 2024-11-12 (Tue)idle

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β˜€: milestone, start, 2024-10-13 00:00:00.000, 0d
β˜ͺ: milestone, end, 2024-10-13 23:59:59.999, 0d

FULL DAY IDLE

No DRAGEN run on 2024-11-12.

4.1.4 2024-11-13 (Wed)

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β˜€: milestone, start, 2024-10-13 00:00:00.000, 0d
wgs420_10 (31m 25s): 2024-10-13 15:49:24.996,2024-10-13 16:21:00.067
wgs420_4 (28m 29s): 2024-10-13 15:49:25.207,2024-10-13 16:18:10.359
wgs419_24 (30m 30s): 2024-10-13 16:18:06.907,2024-10-13 16:48:56.961
wgs419_17 (43m 16s): 2024-10-13 16:21:06.997,2024-10-13 17:04:17.099
wgs419_13 (30m 7s): 2024-10-13 16:48:22.655,2024-10-13 17:18:47.704
wgs419_2 (37m 53s): 2024-10-13 17:04:24.218,2024-10-13 17:42:04.344
wgs419_18 (31m 24s): 2024-10-13 17:19:22.074,2024-10-13 17:50:22.131
wgs419_14 (35m 51s): 2024-10-13 17:42:12.558,2024-10-13 18:18:02.671
wgs419_19 (37m 15s): 2024-10-13 17:50:19.042,2024-10-13 18:27:24.242
wgs419_48 (36m 17s): 2024-10-13 18:18:21.969,2024-10-13 18:54:12.035
wgs420_6 (29m 16s): 2024-10-13 18:27:19.332,2024-10-13 18:56:54.385
wgs419_31 (36m 6s): 2024-10-13 18:54:44.862,2024-10-13 19:30:34.982
wgs419_32 (42m 19s): 2024-10-13 19:30:30.552,2024-10-13 20:12:45.622
wgs420_5 (37m 4s): 2024-10-13 19:44:35.677,2024-10-13 20:22:10.745
wgs420_9 (43m 17s): 2024-10-13 20:12:40.509,2024-10-13 20:56:20.579
wgs419_41 (46m 11s): 2024-10-13 20:22:24.381,2024-10-13 21:08:09.672
wgs419_4 (37m 17s): 2024-10-13 20:55:57.604,2024-10-13 21:33:12.667
wgs420_18 (42m 23s): 2024-10-13 21:33:34.756,2024-10-13 22:15:49.876
wgs419_11 (38m 28s): 2024-10-13 21:47:17.389,2024-10-13 22:25:37.508
wgs419_12 (50m 54s): 2024-10-13 22:16:08.086,2024-10-13 23:07:03.181
wgs420_8 (1h 14m 43s): 2024-10-13 22:25:30.317,2024-10-13 23:40:35.438
wgs419_45 (47m 35s): 2024-10-13 23:06:50.871,2024-10-13 23:54:15.946
wgs419_43 (39m 2s): 2024-10-13 23:40:19.022,2024-10-14 00:19:23.963
wgs419_34 (58m 10s): 2024-10-13 23:54:14.168,2024-10-14 00:52:34.258
β˜ͺ: milestone, end, 2024-10-13 23:59:59.999, 0d

4.1.5 2024-11-14 (Thu)

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β˜€: milestone, start, 2024-10-14 00:00:00.000, 0d
wgs419_28 (45m 31s): 2024-10-14 00:19:11.956,2024-10-14 01:05:02.294
wgs419_33 (37m 48s): 2024-10-14 00:52:49.423,2024-10-14 01:30:14.482
wgs419_8 (33m 21s): 2024-10-14 01:30:42.768,2024-10-14 02:04:02.822
wgs420_13 (35m 30s): 2024-10-14 01:43:08.565,2024-10-14 02:18:38.626
wgs419_16 (37m 31s): 2024-10-14 02:03:29.059,2024-10-14 02:41:14.176
wgs420_11 (35m 42s): 2024-10-14 02:18:26.553,2024-10-14 02:54:16.835
wgs419_36 (38m 14s): 2024-10-14 02:41:06.994,2024-10-14 03:19:27.058
wgs419_40 (37m 32s): 2024-10-14 02:54:17.090,2024-10-14 03:31:22.158
wgs419_10 (33m 39s): 2024-10-14 03:19:29.987,2024-10-14 03:53:10.050
wgs419_29 (40m 19s): 2024-10-14 03:31:30.356,2024-10-14 04:12:05.424
wgs419_6 (39m 30s): 2024-10-14 03:53:36.875,2024-10-14 04:32:46.927
wgs420_15 (37m 50s): 2024-10-14 04:11:47.328,2024-10-14 04:50:07.389
wgs419_15 (39m 13s): 2024-10-14 04:32:37.453,2024-10-14 05:11:57.499
wgs419_27 (36m 56s): 2024-10-14 04:49:42.535,2024-10-14 05:26:32.581
wgs419_1 (36m 15s): 2024-10-14 05:11:59.731,2024-10-14 05:48:19.795
wgs419_25 (34m 28s): 2024-10-14 05:26:40.016,2024-10-14 06:01:10.461
wgs419_22 (39m 5s): 2024-10-14 05:48:15.807,2024-10-14 06:27:05.866
wgs419_37 (37m 18s): 2024-10-14 06:07:46.129,2024-10-14 06:44:21.424
wgs419_20 (39m 22s): 2024-10-14 06:27:01.570,2024-10-14 07:06:36.690
wgs419_30 (38m 2s): 2024-10-14 06:44:22.482,2024-10-14 07:22:12.559
wgs419_26 (38m 29s): 2024-10-14 07:06:49.308,2024-10-14 07:45:09.382
wgs419_44 (36m 22s): 2024-10-14 07:22:56.983,2024-10-14 07:58:57.050
wgs419_21 (33m 41s): 2024-10-14 07:45:39.768,2024-10-14 08:18:44.883
wgs419_47 (30m 2s): 2024-10-14 07:58:37.621,2024-10-14 08:28:52.659
wgs419_46 (41m 7s): 2024-10-14 08:19:22.650,2024-10-14 08:59:57.702
wgs419_42 (33m 53s): 2024-10-14 08:28:56.784,2024-10-14 09:02:31.831
wgs419_35 (46m 38s): 2024-10-14 09:00:01.929,2024-10-14 09:46:41.997
wgs419_39 (37m): 2024-10-14 09:02:54.953,2024-10-14 09:39:45.017
wgs419_9 (43m 39s): 2024-10-14 09:40:02.906,2024-10-14 10:23:22.971
wgs419_23 (40m 5s): 2024-10-14 09:52:47.495,2024-10-14 10:32:37.661
wgs419_38 (33m 28s): 2024-10-14 10:23:59.829,2024-10-14 10:56:45.008
wgs420_3 (37m 39s): 2024-10-14 10:32:39.812,2024-10-14 11:10:09.878
wgs419_7 (38m 55s): 2024-10-14 10:57:14.761,2024-10-14 11:35:55.091
β˜ͺ: milestone, end, 2024-10-14 23:59:59.999, 0d

4.1.6 2024-11-15 (Fri)idle

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β˜€: milestone, start, 2024-10-15 00:00:00.000, 0d
wgs420_7 (32m 59s): 2024-10-15 17:02:28.156,2024-10-15 17:35:38.240
wgs420_12 (26m 45s): 2024-10-15 17:02:28.239,2024-10-15 17:29:13.338
β˜ͺ: milestone, end, 2024-10-15 23:59:59.999, 0d

Caution

Just 2 DRAGEN runs on 2024-11-12.

4.1.7 2024-11-16 (Sat)

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β˜€: milestone, start, 2024-10-16 00:00:00.000, 0d
2024#45;10#45;16 18#58;27#58;07: milestone, 2024-10-16 18:27:07.499, 0d
wgs420_35 (29m 12s): 2024-10-16 18:27:07.499,2024-10-16 18:56:32.640
wgs418_65 (26m 26s): 2024-10-16 18:27:35.331,2024-10-16 18:54:05.762
wgs420_40 (31m 39s): 2024-10-16 18:56:20.058,2024-10-16 19:28:25.124
wgs420_34 (28m 10s): 2024-10-16 19:25:54.979,2024-10-16 19:54:25.016
wgs420_20 (30m 54s): 2024-10-16 19:28:11.705,2024-10-16 19:59:16.749
wgs420_24 (35m 19s): 2024-10-16 19:54:00.734,2024-10-16 20:29:40.951
wgs420_19 (35m 5s): 2024-10-16 19:58:58.731,2024-10-16 20:34:18.775
wgs420_43 (31m 53s): 2024-10-16 20:29:28.706,2024-10-16 21:01:23.901
wgs420_45 (36m 32s): 2024-10-16 20:34:19.136,2024-10-16 21:10:54.275
wgs420_25 (33m 52s): 2024-10-16 21:01:33.353,2024-10-16 21:35:23.404
wgs420_42 (37m 36s): 2024-10-16 21:10:39.276,2024-10-16 21:48:09.337
wgs418_45 (43m 33s): 2024-10-16 21:35:30.602,2024-10-16 22:18:55.666
wgs420_36 (38m 3s): 2024-10-16 21:48:20.861,2024-10-16 22:26:25.926
wgs418_66 (35m 40s): 2024-10-16 22:19:01.991,2024-10-16 22:54:42.066
wgs418_46 (39m 11s): 2024-10-16 22:26:27.475,2024-10-16 23:05:27.538
wgs420_33 (50m 52s): 2024-10-16 22:54:47.854,2024-10-16 23:45:27.921
wgs420_26 (48m 40s): 2024-10-16 23:05:29.337,2024-10-16 23:54:14.474
wgs420_41 (33m 38s): 2024-10-16 23:45:51.300,2024-10-17 00:19:11.366
β˜ͺ: milestone, end, 2024-10-16 23:59:59.999, 0d

4.1.8 2024-11-17 (Sun)full

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β˜€: milestone, start, 2024-10-17 00:00:00.000, 0d
wgs418_3 (38m 53s): a1, 2024-10-17 00:00:47.017,2024-10-17 00:39:52.157
wgs420_93 (44m 3s): 2024-10-17 00:19:05.641,2024-10-17 01:03:15.934
wgs420_14 (36m 52s): 2024-10-17 00:39:47.931,2024-10-17 01:16:43.009
wgs418_68 (34m 12s): 2024-10-17 01:03:08.468,2024-10-17 01:37:23.855
wgs420_69 (36m 1s): 2024-10-17 01:16:35.519,2024-10-17 01:52:55.594
wgs420_65 (49m 46s): 2024-10-17 01:37:11.450,2024-10-17 02:27:16.554
wgs420_64 (33m 19s): 2024-10-17 01:52:47.447,2024-10-17 02:25:57.555
wgs420_66 (32m 28s): 2024-10-17 02:26:19.581,2024-10-17 02:58:54.708
wgs420_70 (28m 53s): 2024-10-17 02:26:56.152,2024-10-17 02:55:51.221
wgs420_1 (35m 51s): 2024-10-17 02:56:23.198,2024-10-17 03:32:03.244
wgs420_81 (34m 25s): 2024-10-17 02:58:51.455,2024-10-17 03:33:11.503
wgs420_28 (28m 5s): 2024-10-17 03:32:04.223,2024-10-17 03:59:54.259
wgs420_39 (30m 19s): 2024-10-17 03:32:59.516,2024-10-17 04:03:14.555
wgs420_44 (39m 31s): 2024-10-17 04:00:04.680,2024-10-17 04:39:54.750
wgs420_32 (31m 36s): 2024-10-17 04:03:25.563,2024-10-17 04:34:50.627
wgs420_30 (29m 25s): 2024-10-17 04:39:55.960,2024-10-17 05:08:46.005
wgs420_31 (32m 46s): 2024-10-17 04:41:10.929,2024-10-17 05:14:15.974
wgs418_67 (32m 2s): 2024-10-17 05:09:31.110,2024-10-17 05:41:11.168
wgs420_23 (37m 20s): 2024-10-17 05:14:16.553,2024-10-17 05:51:46.615
wgs420_49 (33m 33s): 2024-10-17 05:40:56.600,2024-10-17 06:14:31.769
wgs420_29 (35m 41s): 2024-10-17 05:51:26.815,2024-10-17 06:27:11.859
wgs420_48 (40m 37s): 2024-10-17 06:14:48.182,2024-10-17 06:55:28.414
wgs418_62 (39m 34s): 2024-10-17 06:27:03.354,2024-10-17 07:07:03.454
wgs420_21 (34m 32s): 2024-10-17 06:55:29.113,2024-10-17 07:29:44.416
wgs420_46 (38m 33s): 2024-10-17 07:06:34.145,2024-10-17 07:45:14.272
wgs420_37 (32m 48s): 2024-10-17 07:30:04.259,2024-10-17 08:02:39.322
wgs420_47 (39m 33s): 2024-10-17 07:45:10.473,2024-10-17 08:24:45.534
wgs418_69 (38m 15s): 2024-10-17 08:08:27.025,2024-10-17 08:46:37.417
wgs420_22 (35m 43s): 2024-10-17 08:24:42.153,2024-10-17 09:00:47.223
wgs418_86 (1h 2m 37s): 2024-10-17 08:46:31.947,2024-10-17 09:49:02.200
wgs418_89 (41m 13s): 2024-10-17 09:00:30.394,2024-10-17 09:42:05.467
wgs418_71 (31m 39s): 2024-10-17 09:41:45.341,2024-10-17 10:13:45.391
wgs418_94 (38m 44s): 2024-10-17 09:48:58.445,2024-10-17 10:27:48.523
wgs418_80 (40m 31s): 2024-10-17 10:13:55.133,2024-10-17 10:54:15.188
wgs418_77 (40m 24s): 2024-10-17 10:28:01.286,2024-10-17 11:08:36.338
wgs418_87 (51m 59s): 2024-10-17 10:54:26.325,2024-10-17 11:46:01.406
wgs418_95 (38m 17s): 2024-10-17 11:08:06.469,2024-10-17 11:46:31.531
wgs418_76 (37m 48s): 2024-10-17 11:45:57.380,2024-10-17 12:24:07.431
wgs418_70 (36m 19s): 2024-10-17 11:46:43.171,2024-10-17 12:23:03.235
wgs418_93 (40m 12s): 2024-10-17 12:23:48.542,2024-10-17 13:04:13.661
wgs418_78 (37m 46s): 2024-10-17 12:29:28.216,2024-10-17 13:07:18.276
wgs418_92 (48m 47s): 2024-10-17 13:04:16.508,2024-10-17 13:52:51.641
wgs418_84 (42m 41s): 2024-10-17 13:07:33.293,2024-10-17 13:49:58.365
wgs418_75 (1h 1m 23s): 2024-10-17 13:50:15.051,2024-10-17 14:51:30.161
wgs418_88 (57m 39s): 2024-10-17 13:52:48.854,2024-10-17 14:50:23.949
wgs418_81 (48m 16s): 2024-10-17 14:50:49.637,2024-10-17 15:38:44.790
wgs418_73 (45m 13s): 2024-10-17 14:51:36.305,2024-10-17 15:36:36.385
wgs418_90 (49m 9s): 2024-10-17 15:37:05.725,2024-10-17 16:25:55.795
wgs418_82 (48m 28s): 2024-10-17 15:39:15.275,2024-10-17 16:27:10.417
wgs418_79 (39m 54s): 2024-10-17 16:25:42.904,2024-10-17 17:05:47.967
wgs418_85 (40m 56s): 2024-10-17 16:27:15.870,2024-10-17 17:08:20.952
wgs418_83 (57m): 2024-10-17 17:05:59.853,2024-10-17 18:02:44.955
wgs418_74 (55m 48s): 2024-10-17 17:08:42.535,2024-10-17 18:04:07.624
wgs418_72 (37m 12s): 2024-10-17 18:02:43.759,2024-10-17 18:40:08.822
wgs418_91 (42m 43s): 2024-10-17 18:04:34.591,2024-10-17 18:46:49.664
wgs420_60 (56m 26s): 2024-10-17 18:40:17.421,2024-10-17 19:36:27.707
wgs420_52 (41m 14s): 2024-10-17 18:46:58.514,2024-10-17 19:28:03.594
wgs420_51 (42m 15s): 2024-10-17 19:28:00.440,2024-10-17 20:10:20.519
wgs420_55 (38m): 2024-10-17 19:36:38.212,2024-10-17 20:14:43.275
wgs420_54 (41m 59s): 2024-10-17 20:10:32.485,2024-10-17 20:52:22.606
wgs420_71 (35m 45s): 2024-10-17 20:14:27.711,2024-10-17 20:50:37.765
wgs420_68 (31m 42s): 2024-10-17 20:50:26.752,2024-10-17 21:22:21.930
wgs420_62 (34m 40s): 2024-10-17 20:52:19.088,2024-10-17 21:27:09.202
wgs420_56 (33m 25s): 2024-10-17 21:22:37.912,2024-10-17 21:55:23.141
wgs420_63 (38m 33s): 2024-10-17 21:27:34.940,2024-10-17 22:05:50.022
wgs420_53 (33m 15s): 2024-10-17 21:55:50.918,2024-10-17 22:28:35.984
wgs420_59 (39m 25s): 2024-10-17 22:06:03.455,2024-10-17 22:44:53.513
wgs420_72 (35m 30s): 2024-10-17 22:29:01.504,2024-10-17 23:04:36.543
wgs420_61 (36m 53s): 2024-10-17 22:45:10.032,2024-10-17 23:21:55.093
wgs420_50 (35m 45s): 2024-10-17 23:04:57.219,2024-10-17 23:40:12.277
wgs420_57 (40m 38s): 2024-10-17 23:21:46.990,2024-10-18 00:02:52.058
wgs420_58 (37m 26s): 2024-10-17 23:40:36.801,2024-10-18 00:17:41.851
β˜ͺ: milestone, end, 2024-10-17 23:59:59.999, 0d

FULL DAY RUN

A full day of DRAGEN runs on 2024-11-17.

4.1.9 2024-11-18 (Mon)

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β˜€: milestone, start, 2024-10-18 00:00:00.000, 0d
wgs420_75 (49m 21s): 2024-10-18 00:03:04.798,2024-10-18 00:51:59.863
wgs420_79 (37m 12s): 2024-10-18 00:17:43.082,2024-10-18 00:54:38.342
wgs420_90 (37m 20s): 2024-10-18 00:51:47.403,2024-10-18 01:29:12.476
wgs420_76 (38m 46s): 2024-10-18 00:54:53.754,2024-10-18 01:33:28.977
wgs420_92 (31m 19s): 2024-10-18 01:29:13.876,2024-10-18 02:00:53.973
wgs420_80 (29m 52s): 2024-10-18 01:33:18.137,2024-10-18 02:03:18.348
wgs420_77 (32m 48s): 2024-10-18 02:01:05.829,2024-10-18 02:33:25.883
wgs420_85 (30m 11s): 2024-10-18 02:03:17.518,2024-10-18 02:33:42.566
wgs420_87 (28m 47s): 2024-10-18 02:33:19.592,2024-10-18 03:02:24.630
wgs420_78 (34m 2s): 2024-10-18 02:33:37.261,2024-10-18 03:07:27.383
wgs420_86 (30m 59s): 2024-10-18 03:02:35.547,2024-10-18 03:33:15.601
wgs420_67 (31m 7s): 2024-10-18 03:08:03.200,2024-10-18 03:38:58.310
wgs420_91 (33m 2s): 2024-10-18 03:33:51.249,2024-10-18 04:06:21.320
wgs420_82 (31m 30s): 2024-10-18 03:38:53.431,2024-10-18 04:10:23.462
wgs420_88 (32m 13s): 2024-10-18 04:06:33.444,2024-10-18 04:38:48.669
wgs420_89 (37m 39s): 2024-10-18 04:10:25.560,2024-10-18 04:47:40.635
wgs420_74 (39m 20s): 2024-10-18 04:38:39.962,2024-10-18 05:18:00.032
wgs420_83 (35m 54s): 2024-10-18 04:48:22.286,2024-10-18 05:23:37.426
wgs420_84 (31m 18s): 2024-10-18 05:18:01.237,2024-10-18 05:49:21.293
wgs420_2 (32m): 2024-10-18 05:23:51.720,2024-10-18 05:55:51.926
wgs420_27 (30m 13s): 2024-10-18 05:49:47.611,2024-10-18 06:19:37.832
wgs420_73 (38m 8s): 2024-10-18 05:55:36.785,2024-10-18 06:33:46.892
wgs420_17 (30m 2s): 2024-10-18 06:19:25.875,2024-10-18 06:49:40.910
β˜ͺ: milestone, end, 2024-10-18 23:59:59.999, 0d

4.1.10 2024-11-19 (Tue)idle

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β˜€: milestone, start, 2024-10-13 00:00:00.000, 0d
β˜ͺ: milestone, end, 2024-10-13 23:59:59.999, 0d

FULL DAY IDLE

No DRAGEN run on 2024-11-12.

4.1.11 2024-11-20 (Wed)idle

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β˜ͺ: milestone, end, 2024-10-13 23:59:59.999, 0d

FULL DAY IDLE

No DRAGEN run on 2024-11-12.

4.1.12 2024-11-21 (Thu)

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β˜€: milestone, start, 2024-10-21 00:00:00.000, 0d
wgs421_35 (32m 2s): 2024-10-21 10:00:48.688,2024-10-21 10:32:58.770
wgs421_12 (25m 51s): 2024-10-21 10:00:49.452,2024-10-21 10:27:04.517
wgs421_36 (31m 56s): 2024-10-21 10:27:24.569,2024-10-21 10:58:49.634
wgs421_18 (41m 11s): 2024-10-21 10:33:24.678,2024-10-21 11:13:59.761
wgs421_34 (50m 30s): 2024-10-21 10:58:34.796,2024-10-21 11:49:34.893
wgs421_16 (39m 12s): 2024-10-21 11:13:59.931,2024-10-21 11:53:25.059
wgs421_14 (32m 5s): 2024-10-21 11:49:45.384,2024-10-21 12:21:10.450
wgs422_83 (39m 3s): 2024-10-21 11:53:30.705,2024-10-21 12:32:25.819
wgs421_40 (39m 6s): 2024-10-21 12:21:44.231,2024-10-21 13:00:39.477
wgs422_16 (40m 43s): 2024-10-21 12:32:36.804,2024-10-21 13:13:21.885
wgs422_17 (35m 4s): 2024-10-21 13:00:37.606,2024-10-21 13:35:32.664
wgs421_37 (39m 36s): 2024-10-21 13:13:17.896,2024-10-21 13:53:03.005
wgs422_77 (38m 14s): 2024-10-21 13:41:41.609,2024-10-21 14:19:16.661
wgs421_39 (33m 59s): 2024-10-21 13:52:52.306,2024-10-21 14:26:57.361
wgs422_19 (33m 15s): 2024-10-21 14:25:05.460,2024-10-21 14:58:10.648
wgs421_20 (38m 3s): 2024-10-21 14:27:32.533,2024-10-21 15:04:47.597
wgs421_38 (30m 31s): 2024-10-21 14:57:54.444,2024-10-21 15:28:49.468
wgs421_24 (42m 24s): 2024-10-21 15:05:05.725,2024-10-21 15:47:25.863
wgs422_78 (36m 8s): 2024-10-21 15:29:06.283,2024-10-21 16:04:36.361
wgs421_26 (41m 16s): 2024-10-21 15:47:07.854,2024-10-21 16:28:37.922
wgs421_33 (30m 20s): 2024-10-21 16:33:49.356,2024-10-21 17:04:09.418
wgs421_17 (38m 40s): 2024-10-21 17:03:59.559,2024-10-21 17:42:19.629
wgs422_79 (30m 29s): 2024-10-21 17:42:28.609,2024-10-21 18:13:08.670
wgs421_32 (35m 34s): 2024-10-21 18:12:53.899,2024-10-21 18:48:28.963
wgs421_25 (34m 47s): 2024-10-21 18:13:38.912,2024-10-21 18:48:23.971
wgs421_42 (41m 25s): 2024-10-21 18:48:16.695,2024-10-21 19:29:51.779
wgs421_43 (39m 25s): 2024-10-21 20:00:05.479,2024-10-21 20:39:15.551
wgs421_30 (35m 17s): 2024-10-21 20:39:35.620,2024-10-21 21:14:25.736
wgs421_23 (40m 40s): 2024-10-21 21:08:40.877,2024-10-21 21:49:10.939
wgs421_29 (36m 48s): 2024-10-21 21:49:01.668,2024-10-21 22:25:56.725
wgs421_28 (37m 11s): 2024-10-21 22:28:17.198,2024-10-21 23:05:47.241
wgs422_15 (34m 58s): 2024-10-21 22:37:37.349,2024-10-21 23:12:22.400
wgs421_21 (37m 28s): 2024-10-21 23:06:03.150,2024-10-21 23:43:08.202
wgs421_27 (36m 19s): 2024-10-21 23:12:24.441,2024-10-21 23:48:49.487
β˜ͺ: milestone, end, 2024-10-21 23:59:59.999, 0d